Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility
-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Kolluri Kanti
Sent: 18 March 2020 05:54
To: [hidden email] Subject: Stackreg zero padding issue
We use Stackreg to align an image stack and try to apply the transformation matrices obtained to another channel of the same image stack. We do rigid registration of the image stack and stackreg does great. However, it also does zero padding of some of the images in the stack. Now, when we apply the transformation matrices (localTransform) obtained from StackReg to another channel of the same stack, this zero padding does not happen and so although the transformation matrices are applied, the 2 image stacks are not aligned well.