3D projections

Posted by Knecht, David on Mar 30, 2020; 7:53pm
URL: http://imagej.273.s1.nabble.com/3D-projections-tp5023114.html

I have a confocal data stack where the voxels are 0.06 x 0.06 x 0.5.  When Z projected at a 90° angle, you get a series of interdigitated white and black lines unless you interpolate.  I am curious as to why the data is represented this way.  If the sections are 0.5µm thick, and stacked upon each other, where is the black space between coming from?  I would have expected that looking from the side, you would see a white smear without a lot of surface depth cueing.  Thanks- Dave

Dr. David Knecht
Professor, Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
860-486-2200


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