Re: 3D projections

Posted by Joel Sheffield on
URL: http://imagej.273.s1.nabble.com/3D-projections-tp5023114p5023115.html

This is just a guess, but I assume that ImageJ considers each slice to be
essentially infinitely thin, spaced at the step distance.


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
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On Mon, Mar 30, 2020 at 3:53 PM Knecht, David <[hidden email]>
wrote:

> I have a confocal data stack where the voxels are 0.06 x 0.06 x 0.5.  When
> Z projected at a 90° angle, you get a series of interdigitated white and
> black lines unless you interpolate.  I am curious as to why the data is
> represented this way.  If the sections are 0.5µm thick, and stacked upon
> each other, where is the black space between coming from?  I would have
> expected that looking from the side, you would see a white smear without a
> lot of surface depth cueing.  Thanks- Dave
>
> Dr. David Knecht
> Professor, Department of Molecular and Cell Biology
> University of Connecticut
> 91 N. Eagleville Rd.
> U-3125
> Storrs, CT 06269-3125
> 860-486-2200
>
>
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