Quantification of intranuclear label – immunocytochemistry

Posted by bcha7650 on
URL: http://imagej.273.s1.nabble.com/Quantification-of-intranuclear-label-immunocytochemistry-tp5023699.html

I have performed immunocytochemistry on an antigen which is both cytoplasmic
and intranuclear and would like to quantify the intensity density of
intranuclear label. I have labelled the nuclei with DAPI.

The workflow I have attempted was to first split the colours, threshold the
DAPI with outlines showing the ROIs then attempt to create a mask. However,
I am not sure how to overlay the mask in the other channel so that I can
measure the intensity density of the red channel using the masks I created
in the blue channel.

Have I got the workflow correct, or is there a better way to do this?

Any help is greatly appreciated

B







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