http://imagej.273.s1.nabble.com/Find-Maxima-segmentation-macro-issue-tp5023746p5023747.html
Please post an image and a short macro so others can try to reproduce the
> Hi all,
>
> I've run into an issue when running the "Find Maxima..." segmentation
> algorithm by Michael Schmid: Basically, running the segmentation from a
> macro produces different results than a manual run - from the same image,
> with the same parameters provided.
>
> Parameters: "prominence=32767 strict above output=[Segmented Particles]"
>
> The image is a 500x500 px overlay of nuclei (values: 0, 65535) and cytosol
> markers (values: 0-32767), which, in principle, works as a marker-based
> segmentation - when run manually. But it looks like a marco run produces
> additional particles, which are lines with a width of single pixels. I've
> tried to exclude those additional particles with the Analyze Particles...
> with size or circularity limits, but unfortunately that also excludes valid
> results (particles derived from maxima).
>
> Could I run the "Find Maxima..." segmentation from the macro in Java or JS
> as a workaround / for testing? (I've consulted the ImageJ documentation,
> but unfortunately my understanding of ImageJ is still too limited.)
>
> I'd like to troubleshoot this issue, since it's currently a roadblock in my
> image processing pipeline.
>
>
> Thanks in advance,
> Christian R.
>
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