Posted by
Christian R.-2 on
URL: http://imagej.273.s1.nabble.com/Find-Maxima-segmentation-macro-issue-tp5023746p5023753.html
Hi Michael,
I've only seen your response after my update. - Thanks for taking time to
brainstorm about my issue,
and of course for anwering my questions!
Yes, the issue was puzzling me - quite a bit. I thought that maybe a
parameter might not've been transferred to the (Java) function call from the
macro to the dialog or that maybe there was some issue with the numerical
representation of these parameters.
But actual the cause was an oversight of mine:
With the the combined nuclei/cytosol image, my macro ran an additional
watershed step (unintentionally) *after* I had confirmed the number of
maxima (using the "Find Maxima..." function) but *before* calling the
"Analyze Particle" function to count the number of maker-based segmented
particles. This would always cause too many particles to being produced.
If I then went back to segment the particles of the combined image manually,
the watershed step would be skipped and the number of nuclei and cytosol
would match perfectly.
The Find Maxima... segmentation works nicely and I'm very glad it's publicly
available in ImageJ.
If I can return the favor and help with testing or the documention, please
do not hesitate to contact me!
Chris
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