Re: Gaussian blur before or after Background correction?

Posted by Herbie on Aug 01, 2020; 7:33am
URL: http://imagej.273.s1.nabble.com/Gaussian-blur-before-or-after-Background-correction-tp5023797p5023798.html

Greetings Thomas,

before commenting your a-c questions it would be important to understand

A) what "the gain was set too high" really means (overexposure?, clipping?)

B) why you think a Gaussian lowpass will or can compensate for the high
gain effects.

Best

Herbie

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Am 31.07.20 um 22:32 schrieb Thomas Fischer:

> Hi everybody,
>
> In some of my (microscopic) images the gain was set too high. I have to
> apply a Gaussian blur. I also have empty images and plan to apply a
> Background correction by division. Should I apply the Gaussian blur before
> (a) or after (b) Background correction?
> (a) Gaussian blur applied to the image and empty image separately
> (b) Gaussian blur applied to the corrected image
>
> Thank you, Thomas
>
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