http://imagej.273.s1.nabble.com/14-bit-NEF-raw-Images-tp5023829p5023833.html
slices. Did you adjust B&C?
> Good morning Straatman and Herbie:
> When I open a 14-bit NEF in dcraw (with ImageJ) at any setting (8-bit, 16-bit) in any space (raw, sRGB) i get an image that is not even close to the what the color should be (i.e. which should be green turfgrass shoots) and the image pixel size is 5600 x 3728 instead of 5568 x 3712 which is what original NEF images are .... if i open in bio-formats (with Fiji) I get a nearly black stack image that is 5600 x 3782 .... if I just use "open" in either imagej or fiji I get an image that appears as it should except that it is a thumbnail that is only 160 x 120 px .... which I can not do anything with .....
> What I can do is use the nikon software to convert the NEF to an 8-bit tiff and then proceed with what I want to do which is to convert to LAB stack or HSB stack and measure ..... if I convert the NEF to 16-bit-tiff and I open it it comes back as a stack that I can not convert unless I change it to a RGB color which I assume is 24 bit .....
> what I'm trying to do is measure the "color" of individual grass shoots so as to eventually compare with a spectrophotometer
> Any thoughts? Would I be better off capturing the image as 12 bit NEF? (which I really have not tried yet) as I want as much color information in each pixel as I can achieve ...
>
> William L. Berndt, Ph.D. 7133 Penner Lane. Apt. #40Fort Myers, FL 33907@Dr_Lee_Berndt239.246.0396
>
> -----Original Message-----
> From: Straatman, Kees (Dr.) <
[hidden email]>
> To:
[hidden email]
> Sent: Tue, Aug 11, 2020 5:04 am
> Subject: Re: 14 bit NEF raw Images
>
> Hi Herbie and Lee
>
> I am able to open NEF files in dcraw, as Lee writes. Only difference I see is a slight difference in size of 6016x4016 versus 6000x4000 when opened in Nikon software. Is this what you mean by "image is still not right"?
>
> I am not able to open NEF files correctly using "BIO-Formats Importer" as suggested. I get just a black image. Direct opening in Fiji results in a thumbnail of 160x120 showing the correct image
>
> Best wishes
>
> Kees
>
> ________________________________
> From: Herbie <
[hidden email]>
> Sent: 11 August 2020 09:13
> Subject: Re: 14 bit NEF raw Images
>
> Good day Lee,
>
> there are several aspects of your question
>
> 1.
> Be aware of the fact that 14bit images will usually be opened and
> handled as 16bit images.
>
> 2.
> If you deal with color images, be aware of the fact that ImageJ doesn't
> handle 16bit RGB images but represents them as stacks with three slices
> that are R, G, and B respectively.
>
> 3.
> Be aware of the fact that quite often you need to use the B&C dialog to
> make 16bit images or stacks look as desired.
>
> 4.
> I was able to open a .NEF image using "BIO-Formats Importer" with setting
> View stack with: "Standard ImageJ"
> Stack order: Default
>
> 5.
> If you have successfully opened the image as RGB-stack go to "Color >
> Channels Tool..."
>
> HTH
>
> Herbie
>
> ::::::::::::::::::::::::::::::::::::::::
> Am 11.08.20 um 00:50 schrieb Lee Berndt:
>> Hi:
>> I'm trying to open 14 bit Nikon NEF images with little success .... using Import/Raw the file opens but is very small (160 x 120 px) .... if I use bio-formats the image opens but is not the correct size or color .... dcraw reader also opens it but the image is still not right .... can someone help me please ? what am I doing wrong? Thank you and have a great evening .... Lee
>>
>> William L. Berndt, Ph.D. 7133 Penner Lane. Apt. #40Fort Myers, FL 33907@Dr_Lee_Berndt239.246.0396
>>
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