Image subtraction then using the resulting image

Posted by h97 on
URL: http://imagej.273.s1.nabble.com/Image-subtraction-then-using-the-resulting-image-tp5023935.html

Dear all,

Hope you are well.

I am new to imagej and need some help with a task which is highly
appreciated.

Basically, I need to analyse thousands of images which would be excruciating
to do manually. What my goal is for example i have 500 images(frames).

1. I want to subtract two consecutive images and get the resulting image.
2. Using the resulting image from step 1 i want to make that the reference
for subtracting from frame #3. for example, frame#1-frame#2=img1. Then i
want to do the same with img1-frame#3=img2 and so on for how many number of
frames I have.

Is there any plugin available or macro to do this to automate the process?
if so, can you please tell me how to install or work with the plugin?


Thanks.



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