Re: Image subtraction then using the resulting image

Posted by Herbie on
URL: http://imagej.273.s1.nabble.com/Image-subtraction-then-using-the-resulting-image-tp5023935p5023936.html

Good day Hassan,

what you want to obtain can be done by using an ImageJ-macro or -plugin
that you need to code yourself because I doubt that somebody has written
such code.

What is missing in your description is what you consider being the
result of the operation. Is it a stack containing the images img1 to imgN ?

For macro-coding see:
<https://imagej.nih.gov/ij/developer/macro/macros.html>
<https://imagej.nih.gov/ij/developer/macro/functions.html>

Regards

Herbie

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Am 15.09.20 um 17:45 schrieb h97:

> Dear all,
>
> Hope you are well.
>
> I am new to imagej and need some help with a task which is highly
> appreciated.
>
> Basically, I need to analyse thousands of images which would be excruciating
> to do manually. What my goal is for example i have 500 images(frames).
>
> 1. I want to subtract two consecutive images and get the resulting image.
> 2. Using the resulting image from step 1 i want to make that the reference
> for subtracting from frame #3. for example, frame#1-frame#2=img1. Then i
> want to do the same with img1-frame#3=img2 and so on for how many number of
> frames I have.
>
> Is there any plugin available or macro to do this to automate the process?
> if so, can you please tell me how to install or work with the plugin?
>
>
> Thanks.
>
>
>
> --
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>
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