Posted by
Cammer, Michael-3 on
URL: http://imagej.273.s1.nabble.com/Image-subtraction-then-using-the-resulting-image-tp5023935p5023938.html
I think I understand what you wat to do.
A macro to do this frame by frame is simple but slow. This is much faster.
If you have enough memory.
Duplicate a stack.
Add a frame to the beginning of one stack.
Add a frame to the end of the other stack.
Subtract one from the other or difference them.
Regards-
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
Office: 646-501-0567 Cell: 914-309-3270
[hidden email]
http://nyulmc.org/micros http://microscopynotes.com/
-----Original Message-----
From: h97 <
[hidden email]>
Sent: Tuesday, September 15, 2020 12:31 PM
To:
[hidden email]
Subject: Re: Image subtraction then using the resulting image
[EXTERNAL]
Perhaps i should have been more clear.
I want to save the img1 to imgN then rub a batch analysis to analyse img1 to imgN to get area of particles.
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