Re: Quantifying colocalization and measuring focal adhesion colocalization in puncta
Posted by
Straub, Volko A. (Dr.) on
Oct 03, 2020; 6:41am
URL: http://imagej.273.s1.nabble.com/Quantifying-colocalization-and-measuring-focal-adhesion-colocalization-in-puncta-tp5023989p5023990.html
Dear Grace,
There are many different tools for analysing colocalisation and I can't really recommend one specific one as it very much depends on the precise nature of your images and analysis task. However, NEUBIAS academy has just run an 'Introduction to Colocalization' webinar this week. You can find a recording of the webinar here:
http://eubias.org/NEUBIAS/training-schools/neubias-academy-home/. This will be followed up next week by a webinar on 'Advanced colocalization methods for SMLM and statistical tools for analyzing the spatial distribution of objects'. So, if you are new to colocalisation analysis it might be worth having a look at these.
Good luck,
Volko
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http://eubias.org/NEUBIAS/training-schools/neubias-academy-home/>
NEUBIAS Academy @Home - NEUBIAS: Network of BioImage Analysts<
http://eubias.org/NEUBIAS/training-schools/neubias-academy-home/>
NEUBIAS Academy is a new initiative, aimed to provide sustainable material and activities focused on Training in Bioimage Analysis.NEUBIAS Academy capitalizes on the success of 15 Training Schools (2016-2020) that have supported over 400 trainees (Early Career Scientists, Facility Staff and Bioimage Analysts), but could not satisfy the high and increasing demand (almost 1000 applicants).
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Subject: Quantifying colocalization and measuring focal adhesion colocalization in puncta
Hello!
What is the best way to measure the changes in focal adhesion colocalization? Do you measure the individual puncta, or whole cell fluorescence?
Additionally, does anyone have any recommendations for quantifying the amount of colocalization in immunofluorescence?
Any help or advice in these issues is appreciated!
Grace Ciabattoni
[hidden email]
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