Re: Query regarding image processing

Posted by Herbie on Oct 18, 2020; 12:05pm
URL: http://imagej.273.s1.nabble.com/Query-regarding-image-processing-tp5024079p5024080.html

Greetings Igor,

not sure if I can read your mind and "know what [you] want to do".

First let me state that I doubt that the provided sample image is an
original image from your EM. The bottom legend is oblique...

Please tell us why you have this enormous gradient in the image which is
detrimental for image analyses.

Furthermore, please stay away from lossy image compression, i.e.
JPG-format. Please provide high quality images in the original
uncompressed data format such as PNG or TIFF.

Please describe more detailed what you regard as "big lumps" and what
you call nano-sized particles.

Regards

Herbie

:::::::::::::::::::::::::::::::::::::::::::::
Am 18.10.20 um 12:33 schrieb Igor Makarovsky:
> Dear All,
>
> My name is Igor and I work for a Pharma company. I have a series of SEM images, from which I have to calculate the relative percentage of the Nano-sized particles. You can see a sample of such an image attached. I know what I want to do - to scale the whole image and subtract the big lumps, but I don't exactly know how to do it. Please advise me how to go about it.
>
> Thank you all in advance,
> Best Regards,
> Igor

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