Re: Plot profile not working with "Limit To Threshold"

Posted by Michael Schmid on
URL: http://imagej.273.s1.nabble.com/Plot-profile-not-working-with-Limit-To-Threshold-tp5024250p5024251.html

Hi Michael,

sorry, I can't reproduce problem 1. I tried 8-bit and 32-bit images,
rectangular and straight line selections, images with and without a
threshold. It all works.
Could you post a small macro (preferable with one of the sample images)
that demonstrates the problem?

2. Segmented lines wider than 1 pixels: Yes, this is a know issue.
In such a case the profile uses the Straightener
(Edit>Selection>Straighten), which only works with splines. I had tried
to change this a while ago, but I had got stuck - it is slightly more
complicated than one might imagine...



Michael
________________________________________________________________
On 30.11.20 16:32, Cammer, Michael wrote:

> Would you please help with two Plot Profile questions?
>
>
>    1.  Plot profile is not working with "Limit To Threshold" checked in measurements window.  Is this normal or a bug?  I can write a work-around if this is expected behavior, but wanted to check first.
>    2.  Segmented lines wider than 1 pixel are changed to spline fits when running profile plots.  Is this a change?
>
>
> Thank you!
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

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