Posted by
Cammer, Michael-3 on
URL: http://imagej.273.s1.nabble.com/Plot-profile-not-working-with-Limit-To-Threshold-tp5024250p5024252.html
Thank you for looking at this.
I posted screen snaps of the problem and a sample image:
http://microscopynotes.com/temp/plot_profile_problem/plot_profile_not_limited.pnghttp://microscopynotes.com/temp/plot_profile_problem/plot_profile_set_limited.pnghttp://microscopynotes.com/temp/plot_profile_problem/plot_profile_problem_image.tifBest regards-
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
[hidden email]<mailto:
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________________________________
From: Michael Schmid <
[hidden email]>
Sent: Monday, November 30, 2020 10:43:13 AM
To:
[hidden email]
Subject: Re: Plot profile not working with "Limit To Threshold"
[EXTERNAL]
Hi Michael,
sorry, I can't reproduce problem 1. I tried 8-bit and 32-bit images,
rectangular and straight line selections, images with and without a
threshold. It all works.
Could you post a small macro (preferable with one of the sample images)
that demonstrates the problem?
2. Segmented lines wider than 1 pixels: Yes, this is a know issue.
In such a case the profile uses the Straightener
(Edit>Selection>Straighten), which only works with splines. I had tried
to change this a while ago, but I had got stuck - it is slightly more
complicated than one might imagine...
Michael
________________________________________________________________
On 30.11.20 16:32, Cammer, Michael wrote:
> Would you please help with two Plot Profile questions?
>
>
> 1. Plot profile is not working with "Limit To Threshold" checked in measurements window. Is this normal or a bug? I can write a work-around if this is expected behavior, but wanted to check first.
> 2. Segmented lines wider than 1 pixel are changed to spline fits when running profile plots. Is this a change?
>
>
> Thank you!
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
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