Re: Regroup two pieces of images

Posted by Michael Schmid on Dec 16, 2020; 6:19pm
URL: http://imagej.273.s1.nabble.com/save-Console-window-text-to-a-file-tp5024273p5024281.html

Hi Vu-Long,

there is an Alpha Channel plugin that might do what you need. It works
for 16-bit images, but the alpha channel must be 8 bits or float (32 bits).
   https://imagejdocu.tudor.lu/plugin/utilities/alpha_channel/start

How to use it:
- The alpha channel and the target image must have the same number of
pixels in x&y.
- It is advisable to have the “Keep multiple undo buffers” option
enabled in Edit>Options>Memory & Threads
- Copy the whole source image and paste it on the target image
- Then call the Alpha Channel plugin; use 'preview' to see the result.
The plugin allows you to modify the alpha channel, e.g. for smoother
boundaries or shrink/enlarge it a few pixels.

Michael
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On 16/12/2020 6:01 pm, vu long tran wrote:

> Dear all,
>
> I am using imagej to process my histological images. I would like to join two pieces of tissues that unfortunately fell apart during the cryo-sectioning process. I could use either GIMP or TrakEM2 to put them together. However, GIMP could not recognize 16 bit images. I would like to keep it at 16 bit since I have several others which are in 16-bit. With TrakEM2, I need to remove the background color. But I could not find a function to add an alpha channel in ImageJ as in GIMP to help me really clear it. I can blend them together but the intensity will be reduced and become hard to compare with other sections.
> I hope someone can give me the suggestions to solve this problem.
>
> Thank you all for your time
> Best regards,
>
>
> Vu-Long Tran
>
> PhD, Postdoctoral Researcher
>
> Laboratory of In Vivo Molecular Imaging (IMIV)
> CEA/Institute of Life Sciences/Hospital Service Frédéric Joliot
>

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