Posted by
Cammer, Michael-3 on
Dec 29, 2020; 3:41am
URL: http://imagej.273.s1.nabble.com/tone-curves-tp5024323p5024324.html
We often use gamma to bring up faint fluorescence without saturating the bright areas and make sure to include in the filename when we save the image.
For instance
control004.tif
becomes
control004 med1 gamma0.65.tif
after median and gamma processing.
This is definitely "more scientific" than the massive problem we have of people saturating their raw data to see fine structure or less abundant molecules.
It would be great to have a non-destructive gamma or even something like the interactive curves function in Photoshop, but the methods we are using with gamma or square root work fine.
Cheers-
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016
[hidden email] http://nyulmc.org/micros http://microscopynotes.com/Voice direct only, no text or messages: 1-914-309-3270 and 1-646-501-0567
________________________________
From: Kenneth Sloan <
[hidden email]>
Sent: Monday, December 28, 2020 9:48:43 PM
To:
[hidden email]
Subject: tone curves
[EXTERNAL]
Are there any tools in ImageJ useful for adjusting tone curves? or, is this kind of image processing considered "non-scientific"?
I just had a problem handed to me that required me to use Lightroom - which worked perfectly, except for the overhead of dealing with the Lightroom interface.
It turned out that I needed 5-point control on the tone curve to achieve the desired effect. If it doesn't exist, I might implement something.
--
Kenneth Sloan
[hidden email]
Vision is the art of seeing what is invisible to others.
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