Re: Spherical aberration in Electron photomicrographs

Posted by Saalfeld, Stephan on Jan 07, 2021; 9:56pm
URL: http://imagej.273.s1.nabble.com/Spherical-aberration-in-Electron-photomicrographs-tp5024337p5024338.html

Can you acquire additional images of textured samples on the same
system or do you already have sufficient overlap (>50%)?

Then you could use this protocol:

https://www.youtube.com/watch?v=lPt-WQuniUs

Skip the stack part that is relevant for confocal stacks only and go
directly into the TrakEM2 section around 4:20.

Best,
Stephan


On Thu, 2021-01-07 at 20:56 +0000, Porter, George M.D. wrote:

> Hello,
>
> I am trying to stitch together montages of cardiac myocytes from
> electron micrographs and I find what I believe to be spherical
> aberration in the images (which apparently can happen with a side
> mount camera). Is there a way to correct this aberration in the
> images electronically using ImageJ or any other program? I have tried
> using the Warp Transformation and Lens Correction and Adaptive Wild
> Angle Filters in Photoshop without consistent success.
>
> Thanks,
> George Porter
>
> George A. Porter, Jr., MD, PhD
> Professor
> Chief and Fellowship Director, Division of Cardiology
> Department of Pediatrics
> University of Rochester Medical Center
> 601 Elmwood Ave.
> Box 631
> Rochester, NY  14642
> Phone: [phone]
> Fax: [phone]
> Email: [hidden email]
>
> "Let me say as I've always said, and I will always continue to say,
> that riots are socially destructive and self-defeating. ... But in
> the final analysis, a riot is the language of the unheard. And what
> is it that America has failed to hear? It has failed to hear that the
> plight of the Negro poor has worsened over the last few years. It has
> failed to hear that the promises of freedom and justice have not been
> met. And it has failed to hear that large segments of white society
> are more concerned about tranquility and the status quo than about
> justice, equality, and humanity. And so in a real sense our nation's
> summers of riots are caused by our nation's winters of delay. And as
> long as America postpones justice, we stand in the position of having
> these recurrences of violence and riots over and over again."
> Martin Luther King, Jr., "The Other America"
>
>
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