Reverse density/intensity measurement

Posted by Dipanjana Ghosh on
URL: http://imagej.273.s1.nabble.com/Reverse-density-intensity-measurement-tp5024386.html

Dear all,
I was using mostly confocal images to measure intensities where the signal
comes as bright and the background is dark.
Now when I am opening a western blot image where the signal is dark and
background is bright, it seems the software is still calculating as it does
for the confocal images. What to amend in the set measurement or some other
settings so that it can take care of respective dark or bright signals?
I Hope I can explain my problem. If not please feel free to ask questions.

Thanking you

Regards,
Dipanjana

--
Dr. Dipanjana Ghosh

Prev: Post doctoral Research Fellow
Department of Biological Sciences
National University of Singapore
Singapore

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