http://imagej.273.s1.nabble.com/Reverse-density-intensity-measurement-tp5024386p5024390.html
It worked this way. Thanks a lot.
> Dear Dipanjana,
> Please apologize in the case I'm not correctly understanding your question.
> But simply doing Edit>Invert (or Ctrl + Shift + I) in order to invert your
> image won't it be enough to solve your issue?
> My best regards,
> Philippe
>
> Philippe CARL
> Laboratoire de Bioimagerie et Pathologies
> UMR 7021 CNRS - Université de Strasbourg
> Faculté de Pharmacie
> 74 route du Rhin
> 67401 ILLKIRCH
> Tel : +33(0)3 68 85 42 89
>
> ----- Mail original -----
> De: "Dipanjana Ghosh" <
[hidden email]>
> À: "imagej" <
[hidden email]>
> Envoyé: Lundi 18 Janvier 2021 09:17:58
> Objet: Reverse density/intensity measurement
>
> Dear all,
> I was using mostly confocal images to measure intensities where the signal
> comes as bright and the background is dark.
> Now when I am opening a western blot image where the signal is dark and
> background is bright, it seems the software is still calculating as it does
> for the confocal images. What to amend in the set measurement or some other
> settings so that it can take care of respective dark or bright signals?
> I Hope I can explain my problem. If not please feel free to ask questions.
>
> Thanking you
>
> Regards,
> Dipanjana
>
> --
> Dr. Dipanjana Ghosh
>
> Prev: Post doctoral Research Fellow
> Department of Biological Sciences
> National University of Singapore
> Singapore
>
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