Re: Help to remove background
Posted by
Knecht, David on
Feb 05, 2021; 1:37pm
URL: http://imagej.273.s1.nabble.com/Help-to-remove-background-tp5024436p5024439.html
If this is autofluorescence of some sort, it is often broad wavelength, so one simple way to remove it is to take an image with a different fluorescence channel than your true signal and subtract the background image (noise) from the signal image. Dave
Dr. David Knecht
Professor, Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
On Feb 5, 2021, at 3:54 AM, OBEID Patricia 154904 <
[hidden email]<mailto:
[hidden email]>> wrote:
*Message sent from a system outside of UConn.*
Hello
I try to improve my images before doing an Auto Threshold for the rest of the quantifications.
I have a significant background noise related to the Fibrin gel in which the cells develop.
In the attached images, the arrows point to areas I would like to "erase" and the cells are circled in green.
[cid:image001.jpg@01D6FBA4.EB60B440]
[cid:image005.jpg@01D6FBA4.EB60B440]
Any help will be welcome.
Patricia
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