http://imagej.273.s1.nabble.com/TR-Threshold-doesn-t-apply-because-of-Threshold-not-set-error-tp5024633p5024634.html
Of course, this is of little help because we are not able to test.
try to change the extension (and tell us)...
> Good afternoon all
> I am having a problem that I have never had before ...
> I work from images from Cellomics with a .C01 extension. With Bioformat I search for Cellomics and I can open my images.
> When I do an AutoThreshold on the .C01 image, I have an error when I click Apply: "Threshold is not set".
> Whereas when I do the same on the .tif image everything is fine.
> Can someone help me and tell me what to do to make it work again for .C01 images?
> My imageJ version: 1.53i
> Thank you everyone and have a nice day
> Patricia
>
> PS : I don�t how to send you the .C01 image, it has been rejected because it contains an attachment of type 'APPLICATION/OCTET-STREAM'
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