Re: How to get the grey value of the color colored lines.

Posted by Straub, Volko A. (Dr.) on
URL: http://imagej.273.s1.nabble.com/How-to-get-the-grey-value-of-the-color-colored-lines-tp5024681p5024682.html

Hi,

This looks very much like a gel analysis. Have you looked at the Gel Analysis tool in ImageJ (https://imagejdocu.tudor.lu/gui/analyze/gels). There are also numerous tutorials online describing gel analysis in ImageJ, e.g. https://lukemiller.org/index.php/2010/11/analyzing-gels-and-western-blots-with-image-j/

Good luck,
Volko


-----Original Message-----
From: ABDOULIE JALLOW <[hidden email]>
Sent: 02 June 2021 03:33
To: [hidden email]
Subject: How to get the grey value of the color colored lines.

Hello all,

please do anyone here have an idea of how to go about quantifying the grey value of the stained lines of a lateral flow paper strip? Am using gold nanoparticles and polyclonal antibodies to form the lines to detect mycotoxins in food samples. please any have an idea help me with this, been struggling with this project for the last 6 months. i have attached the image here..

Thank you.

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