temporal smoothing

[POUVREAU SANDRINE]

Hello everyone.
I am looking for help with temporal smoothing in stacks. I am recording time series of XY images with a Zeiss microscope, that I open as stacks with Image J. I am already doing median filtering within one image, but I also would like to do smoothing between adjacent images (temporal smoothing). How can I do that?
Thanks
Sandrine Pouvreau

[lechristophe]

Hi Sandrine,

Have a look at this plugin : Running Z projector by Nico Stuurman

http://valelab.ucsf.edu/~nico/IJplugins/Running_ZProjector.html

There is also a Kalman averaging filter plugin for more complex
temporal averaging.

http://rsbweb.nih.gov/ij/plugins/kalman.html

Cheers,

Christophe


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]



On Mon, Jun 7, 2010 at 12:10, POUVREAU SANDRINE
<[hidden email]> wrote:
> Hello everyone.
> I am looking for help with temporal smoothing in stacks. I am recording time series of XY images with a Zeiss microscope, that I open as stacks with Image J. I am already doing median filtering within one image, but I also would like to do smoothing between adjacent images (temporal smoothing). How can I do that?
> Thanks
> Sandrine Pouvreau
>

[POUVREAU SANDRINE]

Hi
Running Z projector works fine in my case. Thanks again.
Best
Sandrine


-----Message d'origine-----
De : ImageJ Interest Group [mailto:[hidden email]] De la part de Christophe Leterrier
Envoyé : lundi 7 juin 2010 13:00
À : [hidden email]
Objet : Re: temporal smoothing

Hi Sandrine,

Have a look at this plugin : Running Z projector by Nico Stuurman

http://valelab.ucsf.edu/~nico/IJplugins/Running_ZProjector.html

There is also a Kalman averaging filter plugin for more complex
temporal averaging.

http://rsbweb.nih.gov/ij/plugins/kalman.html

Cheers,

Christophe


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]



On Mon, Jun 7, 2010 at 12:10, POUVREAU SANDRINE
<[hidden email]> wrote:
> Hello everyone.
> I am looking for help with temporal smoothing in stacks. I am recording time series of XY images with a Zeiss microscope, that I open as stacks with Image J. I am already doing median filtering within one image, but I also would like to do smoothing between adjacent images (temporal smoothing). How can I do that?
> Thanks
> Sandrine Pouvreau
>