threshold on an area of a picture

[Lena-Christin Conradi]

Hello!

For the following question I would greatly appreciate any help or suggestion:

I need to measure the threshold of a staining in a certain area of some pictures and NOT from the whole picture. Is there anybody who would know how this can be done?

Many thanks in advance,
Lena
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Cammer, Michael]

http://rsb.info.nih.gov/ij/docs/pdfs/examples.pdf

An alternative procedure for measuring areas:
Analyze → Set Measurements, check 'Limit to Threshold'.  After converting to a binary image,
select Analyze → Measure
This procedure is simpler but does not draw an outline of the measured area.

_________________________________________
Michael Cammer, Optical Microscopy Specialist
http://ocs.med.nyu.edu/microscopy
http://microscopynotes.com/
Cell: [phone]

________________________________________
From: ImageJ Interest Group [[hidden email]] on behalf of Lena [[hidden email]]
Sent: Sunday, November 22, 2015 7:42 AM
To: [hidden email]
Subject: threshold on an area of a picture

Hello!

For the following question I would greatly appreciate any help or suggestion:

I need to measure the threshold of a staining in a certain area of some pictures and NOT from the whole picture. Is there anybody who would know how this can be done?

Many thanks in advance,
Lena
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Jacqueline Ross]

Hi Lena,

I'm late into this conversation but I would draw a Region of Interest (ROI) using one of the tools on the tool bar and store it in the ROI Manager (Analyze - Tools - ROI Manager). Then set your measurements (Analyze - Set Measurements) and any threshold you need to define the objects of interest (Image - Adjust threshold) as Michael has mentioned. Then click Measure from the ROI Manager.

Is that what you are looking for?

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Lena
Sent: Monday, 23 November 2015 1:42 a.m.
To: [hidden email]
Subject: threshold on an area of a picture

Hello!

For the following question I would greatly appreciate any help or suggestion:

I need to measure the threshold of a staining in a certain area of some pictures and NOT from the whole picture. Is there anybody who would know how this can be done?

Many thanks in advance,
Lena
--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html