thresholding phase contrast for tracking cell migration

[davince]

Hello everyone
i'm currently doing cell migration study.
we are using fibroblast like cell and image is taken by phase contrast.

But for phase contrast, thresholding is especially difficult.

I use fiji distribution to try to do the processing since i'm thinking about using the stitching function for generating a montage prior to analysing.

I've found that using find edges and then use auto global threshold by triangle algorithm gives me a acceptable result.

My final requirement is to use tracking function like MTrack2 to track the migration path & position and many other measurement of a ROI of a cell through time lapse stacks.

Can anyone give me a hint if you have experience of tracking phase contrast cell images?

What is the best way to do inorder to get the final data i want?
that is to track movement of ROIs of cells throughuot time lapse stacks.
i hope i can threshold to get entire cell perimeter because apart from centroid x and y, i also need fit ellipse parameters.


Thank you very much in advance

Best,
       Paul