Does anybody have experience with imaging the forces of cells as measured by the movements of synthetic micropillars as a cell substrate? For instance, O du Roure "Force mapping in epithelial cell migration" PNAS 102(7) reports "The resolution of the displacements is of the order of 50 nm..." This is measured by imaging cells by brightfield, phase contrast, or Nomarski and the tops of the micropillars the cells sit on form bright spots. The underlying spots move as the cell pulls on the substrate. I was wondering how this can be calculated from images obtained with microscope objectives of N.A. from 0.80 to 1.40. For instance, there seem to be MatLab scripts floating around that do this accurately (and, of course, we'd rather use ImageJ). Any help would be appreciated as we would like to use this method.
Thank you in advance.
Regards,
Michael
________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
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