Hi Muriel,
On way to know is to check the pixel unit of your image, is it pixel or micron ?
To do so:
- open one of your image in ImageJ/Fiji
- within the menu "Image" go to "Properties..."
A window pops-up with many informations regarding your image, and one is "unit of length".
If it's micron , it's easy, your values are micron^2
If it's pixel, you have to calibrate your image, ie get/calculate the pixel/voxel size.
Do not hesitate to contact me if you need further assistance,
Cheers,
Romain
---------------------------------------------------------------
Dr. Romain Guiet
Bioimaging and Optics Platform (PT-BIOP)
Ecole Polytechnique Fédérale de Lausanne (EPFL)
Faculty of Life Sciences
Station 19, AI 0140
CH-1015 Lausanne
Phone: [+4121 69] 39629
http://biop.epfl.ch/---------------------------------------------------------------
________________________________________
De : ImageJ Interest Group [
[hidden email]] de la part de Muriel Desbois [
[hidden email]]
Envoyé : lundi 19 janvier 2015 20:19
À :
[hidden email]
Objet : unit for surface value
Hi,
I would like to know at is the unit for the surface value given using the ROI Manager.
I'm trying to get the surface value of synapses. I have an in vivo fluorescent marker for synapses. I take a z-stack of the signal, then project the maximum intensity to have a single image. Then I circled the different dots and extract the different values using the ROI Manager. For the surface I have value between 0.0001 and 0.001. I'm trying to understand what is the unit of this "surface". I read it is in square pixel but my issue is how the surface can be inferior to 1.
Thank you very much for your help
Muriel
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