z-projections from macro

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z-projections from macro

Glen MacDonald-2
Hello,

Calling the Z Projection command from the Image/Stacks menu creates a  
projection in a new window.  However, calling a Z Projection using the  
Run(...) option converts the source stack into the projection.

Is there a way to call Z-projection from a macro and keep the original  
stack?

thanks,
glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[hidden email]

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The box said "Requires WindowsXP or better", so I bought a Macintosh.
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Re: z-projections from macro

Christine Labno
Hi Glen,

Running ImageJ 1.43f Windows XP, I get the Z-projection in a
new window and keep the original stack when calling from a
macro with either

run("Z Project..."); or

run("Z Project...", "start=1 stop=114 projection=[Max
Intensity]");

So I can't reproduce your problem.  However, an easy work
around for your situation could be to add

run("Duplicate...", "title=stack.tif duplicate");

before your z-projection command line, that would project a
copy of the stack, thereby preserving the original.  Changing
title= would take care of naming issues.

Best,
Christine




---- Original message ----
>Date: Fri, 7 Aug 2009 10:38:36 -0700
>From: Glen MacDonald <[hidden email]>  
>Subject: z-projections from macro  
>To: [hidden email]
>
>Hello,
>
>Calling the Z Projection command from the Image/Stacks menu
creates a  
>projection in a new window.  However, calling a Z Projection
using the  
>Run(...) option converts the source stack into the projection.
>
>Is there a way to call Z-projection from a macro and keep the
original  

>stack?
>
>thanks,
>glen
>
>
>Glen MacDonald
>Core for Communication Research
>Virginia Merrill Bloedel Hearing Research Center
>Box 357923
>University of Washington
>Seattle, WA 98195-7923  USA
>(206) 616-4156
>[hidden email]
>
>******************************************************************************
>The box said "Requires WindowsXP or better", so I bought a
Macintosh.
>******************************************************************************
Christine Labno, Ph.D.
Asst. Technical Director
Light Microscopy Core
University of Chicago
Office of Shared Research Facilities
Abbott 129
(773) 834-9040 (phone)

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