zstack to channels
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A quick question.
I had to perform "images to stack" to put together multiple images. The software things that they are a z-stack. They're actually different channels. How do I change a z-stack to multi-channel stack? I can't do some of the processing (like having different LUT for each slice) because it thinks it's a z-stack. I'm assuming there's a button that I'm missing. Sho Fujisawa Sr. Digital Microscopist Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 417 E. 68th St. Zuckerman Room 1834 New York NY 10065 (212) 639-6667 (646) 888-2186 ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
Re: zstack to channels
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Hi Sho,
Try the 'Make Composite' command (Image -> Color -> Make Composite). Alternatively, rather than using 'Images to Stack' to combine your images, use 'Merge Channels' (Image -> Color -> Merge Channels). Hope this solves your problem, Volko -----Original Message----- From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Sho Fujisawa Sent: 11 August 2016 17:11 To: [hidden email] Subject: zstack to channels A quick question. I had to perform "images to stack" to put together multiple images. The software things that they are a z-stack. They're actually different channels. How do I change a z-stack to multi-channel stack? I can't do some of the processing (like having different LUT for each slice) because it thinks it's a z-stack. I'm assuming there's a button that I'm missing. Sho Fujisawa Sr. Digital Microscopist Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 417 E. 68th St. Zuckerman Room 1834 New York NY 10065 (212) 639-6667 (646) 888-2186 ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
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"Make Composite" works. Great! thank you.
Sho Fujisawa Sr. Digital Microscopist Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 417 E. 68th St. Zuckerman Room 1834 New York NY 10065 (212) 639-6667 (646) 888-2186 -----Original Message----- From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Straub, Volko A. (Dr.) Sent: Thursday, August 11, 2016 12:19 PM To: [hidden email] Subject: Re: zstack to channels Hi Sho, Try the 'Make Composite' command (Image -> Color -> Make Composite). Alternatively, rather than using 'Images to Stack' to combine your images, use 'Merge Channels' (Image -> Color -> Merge Channels). Hope this solves your problem, Volko -----Original Message----- From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Sho Fujisawa Sent: 11 August 2016 17:11 To: [hidden email] Subject: zstack to channels A quick question. I had to perform "images to stack" to put together multiple images. The software things that they are a z-stack. They're actually different channels. How do I change a z-stack to multi-channel stack? I can't do some of the processing (like having different LUT for each slice) because it thinks it's a z-stack. I'm assuming there's a button that I'm missing. Sho Fujisawa Sr. Digital Microscopist Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 417 E. 68th St. Zuckerman Room 1834 New York NY 10065 (212) 639-6667 (646) 888-2186 ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. -- ImageJ mailing list: http://imagej.nih.gov/ij/list.html |
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