Greetings Anat,
if your image is in 16bit RGB format, i.e. 48bit, the image will open in
ImageJ as a stack of three color channels.
If you don't want to apply color weighting, you get the achromatic
version of your image by performing a z-projection of the stack with
option "Average Intensity".
Regards
Herbie
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Am 01.06.21 um 19:29 schrieb Anat Chemerinski:
> Hello,
>
> I am having a hard time converting my 16-bit color image (from immunohistochemistry staining) to grayscale. Does anyone know how to do this?
>
> Thank you!
> Anat
>
> Anat Chemerinski, MD
> Reproductive Endocrinology & Infertility Fellow, PGY-5
> Department of Obstetrics, Gynecology & Women's Health
> Rutgers Biomedical & Health Sciences
>
[hidden email]
>
[hidden email]
> 301-910-6800
>
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