3D Object Counter + Grouped Z project

[KerL]

I am trying to use the 3D object counter to count cells through z-slices. To speed up the plugin, I am using the ImageJ tool Grouped Z-project (Image --> Stacks --> Tools --> Grouped Z-Project) whereby every pair of z-slices are grouped together (their intensities are averaged). However, after doing this the 3D object counter can no longer count the cells through the z-stack, instead it only counts the cells for the 1st slice. How can I fix this problem?

Thank you for your help,

Kevin Liang

[Michael Doube-4]

Hi Kevin,

Try the particle counter in BoneJ, which is substantially faster non-recursive evolution of the original 3D OC.

http://bonej.org/particles

Michael

On 30/03/15 17:05, KerL wrote:

> I am trying to use the 3D object counter to count cells through z-slices. To
> speed up the plugin, I am using the ImageJ tool Grouped Z-project (Image -->
> Stacks --> Tools --> Grouped Z-Project) whereby every pair of z-slices are
> grouped together (their intensities are averaged). However, after doing this
> the 3D object counter can no longer count the cells through the z-stack,
> instead it only counts the cells for the 1st slice. How can I fix this
> problem?
>
> Thank you for your help,
>
> Kevin Liang
>
>
>
> --
> View this message in context: http://imagej.1557.x6.nabble.com/3D-Object-Counter-Grouped-Z-project-tp5012255.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Michael Doube
BPhil BVSc PGCert(Vet Ed) PhD FHEA MRCVS
Lecturer, Comparative Biomedical Sciences
The Royal Veterinary College, University of London
London NW1 0TU
United Kingdom

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@mdoube

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