4D 5D data rendering

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4D 5D data rendering

Knecht, David
Do we have any way yet in ImageJ to generate a tilted projection from  
a 3D over time (4D) data set (other than manually making a projection  
from each time point and then splicing them into a stack)?  Dave


Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)
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Re: 4D 5D data rendering

Bill Mohler
Dave-

We've got a plugin/macro combo that takes selections from a volume
and reconstructs them as X and Y rotations of Max Intensity
Projections for each timepoint, adding stamps for angle and time at
the bottom.  The output stacks are uncompressed QT .mov files, but a
final stage of the program then rolls the whole thing together as a
QTVR movie (uncompressed and jpeg compressed versions) that is easy
to browse either in QuickTime Player or through the QT Player
importer in ImageJ.  We find it very useful in our work and in
sharing these data with others.

We have JUST appeared to have achieved stability with this so that it
now runs cleanly on both win and mac.  I can send you a version now
that is still waiting to have a few helpful features added.  In a
couple of weeks we should have an even more stable version that has
an improved interface.

Which input file-type are you using?

Bill

>Do we have any way yet in ImageJ to generate a tilted projection
>from a 3D over time (4D) data set (other than manually making a
>projection from each time point and then splicing them into a
>stack)?  Dave
>
>
>Dr. David Knecht
>Department of Molecular and Cell Biology
>Co-head Flow Cytometry and Confocal Microscopy Facility
>U-3125
>91 N. Eagleville Rd.
>University of Connecticut
>Storrs, CT 06269
>860-486-2200
>860-486-4331 (fax)


--
-----------------
William A. Mohler
Associate Professor
Dept. of Genetics and Developmental Biology
University of Connecticut Health Center
MC-3301
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Farmington, CT   06030-3301

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