Hi David,
Process>Math>AND does a bitwise AND operation. Except for very special (and rare) applications, it should be used only with binary images, i.e., thresholded images converted to a mask.
Otherwise, your question seems to refer to a colocalization problem. Have a look at the mailinglist or a web search engine for 'ImageJ colocalization' for hints how to do it (I am not a biologist, so I am not the right person to ask bout this).
Michael
________________________________________________________________
On Mar 25, 2013, at 21:07, David Rueda wrote:
> Hi everyone,
>
> I am currently working on some images from a biologist expert. The goal is
> to get only the pixels that overlap between the green and blue channels.
> To do so I apply a AND math operator, a background subtraction and then I
> measure the overall intensity in the image.
> But I wonder if I am doing the good way.
> I know a lot of you have much more experience with biological images (here
> confocal images), and could may be give me some advice about the best way
> to do.
>
> Thank you,
> David
>
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