Diameter of myelinated axons

[Ayman-2]

Hi,
I am trying to measure the diameter of myelinated axons, I don't know how to do that automatically.
I did that manually but it takes time and I have alot of images.
the feret's diameter is not what I need, its bigger than the actual diameter, but I think I can calculate the diameter from area or perimeter.

Regards,
Ayman

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[Cammer, Michael]

If the image can be neatly segmented by thresholding, then each object may be considered a circle based on the area and the diameter calculated. We did this years ago to calculate G ratios.

________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Ayman
Sent: Monday, March 25, 2013 12:51 PM
To: [hidden email]
Subject: Diameter of myelinated axons

Hi,
I am trying to measure the diameter of myelinated axons, I don't know how to do that automatically.
I did that manually but it takes time and I have alot of images.
the feret's diameter is not what I need, its bigger than the actual diameter, but I think I can calculate the diameter from area or perimeter.

Regards,
Ayman

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[Adam Hughes]

Particle analysis also outputs a circularity parameter.  Perhaps you can
come up with some sort of an ad-hoc shape estimation based the effective
area and circularity together.

On Mon, Mar 25, 2013 at 3:46 PM, Cammer, Michael <[hidden email]
> wrote:

> If the image can be neatly segmented by thresholding, then each object may
> be considered a circle based on the area and the diameter calculated. We
> did this years ago to calculate G ratios.
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: [phone]  Cell: [phone]
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Ayman
> Sent: Monday, March 25, 2013 12:51 PM
> To: [hidden email]
> Subject: Diameter of myelinated axons
>
> Hi,
> I am trying to measure the diameter of myelinated axons, I don't know how
> to do that automatically.
> I did that manually but it takes time and I have alot of images.
> the feret's diameter is not what I need, its bigger than the actual
> diameter, but I think I can calculate the diameter from area or perimeter.
>
> Regards,
> Ayman
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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[Ayman-2]

Thanks Adam and Michael for help.
I also want to know how to measure the diameter of the axon, not feret's diameter.

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