Air bubbles - image edit

[julien]

Hi everyone. I'm looking with microscope to an epoxy layer containing air bubbles, and i have to count them.
I've tried to elaborate this image:

trasforming in 8-bit, made adjust in brightness and contrast and tresholding, but without satisfaying result:



as you can see, after tresholding i lost much information, and i want to use "analyze particles" then.

Do you know if there are other proceeds to have a good b&W image, more or less like this:
http://imagej.1557.x6.nabble.com/file/n4956499/J_1_23.jpg

Thank you for your help!

Julien

[Hayward, Anthea]

Hi,
I've fiddled with the image a bit and the bubbles seem clearer to me - I've attached the image I produced. I used the free software Irfanview. I clicked Image and colour correction, then adjusted the settings to suit.
Hope this helps,
Anthea

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of julien
Sent: 10 October 2014 13:58
To: [hidden email]
Subject: Air bubbles - image edit

Hi everyone. I'm looking with microscope to an epoxy layer containing air bubbles, and i have to count them.
I've tried to elaborate this image:
<http://imagej.1557.x6.nabble.com/file/n5009991/1.bmp>

trasforming in 8-bit, made adjust in brightness and contrast and tresholding, but without satisfaying result:

<http://imagej.1557.x6.nabble.com/file/n5009991/1.jpg>

as you can see, after tresholding i lost much information, and i want to use "analyze particles" then.

Do you know if there are other proceeds to have a good b&W image, more or less like this:
http://imagej.1557.x6.nabble.com/file/n4956499/J_1_23.jpg

Thank you for your help!

Julien




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[julien]

thank you anthea! it's better :)
don't you know any way to get only black and white image, like the sample I posted? i mean, no scale of gray...
thanks
J

[Anderson, Charles (DNR)]

Julien

Can you try using a finer saw or polishing the surface to remove the surface irregularities?  A clearer image would be so much easier to analyze.

The white spot in each bubble is handy for visually finding them, but makes analysis trickier, as the spot is sometimes at the edge of the bubble. Lighting from an oblique angle may reduce the glare.

I suggest reading two papers, then experimenting with hdomes and reconstruction using Landini's morphology plugin.

Halkiotis et al. 2007. Automatic detection of clustered microcalcifications in digital mammograms using mathematical morphology and neural networks. Signal Processing 87:1559-1568.

Vincent. 1993. Morphological grayscale reconstruction in image analysis: applications and efficient algorithms.  IEEE Transactions on Image Processing 2(2):176-201.

Charles

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of julien
Sent: Friday, October 10, 2014 7:58 AM
To: [hidden email]
Subject: Air bubbles - image edit

Hi everyone. I'm looking with microscope to an epoxy layer containing air bubbles, and i have to count them.
I've tried to elaborate this image:
<http://imagej.1557.x6.nabble.com/file/n5009991/1.bmp>

trasforming in 8-bit, made adjust in brightness and contrast and tresholding, but without satisfaying result:

<http://imagej.1557.x6.nabble.com/file/n5009991/1.jpg>

as you can see, after tresholding i lost much information, and i want to use "analyze particles" then.

Do you know if there are other proceeds to have a good b&W image, more or less like this:
http://imagej.1557.x6.nabble.com/file/n4956499/J_1_23.jpg

Thank you for your help!

Julien




--
View this message in context: http://imagej.1557.x6.nabble.com/Air-bubbles-image-edit-tp5009991.html
Sent from the ImageJ mailing list archive at Nabble.com.

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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