> Sir, the Analyse>Surface Plot draws a nice graph of my DIBA
> data, but the graph is in Black and white (1), what should I
> do to draw it in color ?
Add a color lookup table to the image, such as those found in the
Image>Lookup Tables menu.
> (2) what should I do to draw it in
> color with shading depending on cutoff values (like less
> than 0.5 - green, more than 0.51 red)
You can create custom lookup tables using the Color>Edit LUT tool.
> (3) what should I do
> to select multiple areas, like wells in a 96 well micro
> plate,
There is a dot blot analysis tutorial at
http://rsb.info.nih.gov/ij/docs/examples/dot-blot/index.html> My Java programming skills are limited to writing
> Hello world only, Thanks so much in advance, the above
> modifications will help me to make up a free online
> diagnostic tool of Plant viral infections for farmers use,
> thanks Samuel, Virologist, Indian Institute of Horticultural
> Research, Bangalore, India
You will also want to check out the 3D Surface Plot plugin at
http://rsb.info.nih.gov/ij/plugins/surface-plot-3d.htmlIt works with all image types and you can interactively adjust
viewing angle, perspective, scale, lighting and smoothing.
-wayne