Analysing x-z and y-z slices

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Analysing x-z and y-z slices

Graham Wright-5
Hello,
I have a z-stack acquired on a Zeiss LSM 510 META confocal of a sample in 2 channels (single time point). I would like to be able to extract x-z and y-z slices so that I can analyse the fluorescence intensity across them. Is this possible and/or does anyone know how to do this in ImageJ?
Thanks in advance for your help,
Graham

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Graham Wright
Microscopy & Imaging Facility

Temasek Life Sciences Laboratory
1 Research Link
National University of Singapore
Singapore 117604

P:    +65 6872 8406
M:   +65 8256 7916
E:     [hidden email]
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Re: Analysing x-z and y-z slices

Fabrice Senger
Graham Wright a écrit :

> Hello,
> I have a z-stack acquired on a Zeiss LSM 510 META confocal of a sample in 2 channels (single time point). I would like to be able to extract x-z and y-z slices so that I can analyse the fluorescence intensity across them. Is this possible and/or does anyone know how to do this in ImageJ?
> Thanks in advance for your help,
> Graham
>
> -----
> Graham Wright
> Microscopy & Imaging Facility
>
> Temasek Life Sciences Laboratory
> 1 Research Link
> National University of Singapore
> Singapore 117604
>
> P:    +65 6872 8406
> M:   +65 8256 7916
> E:     [hidden email]
>  
you may try with volume viewer.

cheers,

fabrice.

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Senger Fabrice