Background correction

[Brittany Oliva]

Hello,
     I have a question that I cannot find information for on the  
website.  I have an image which has a background that I want to get  
rid of, but it's an abnormal background.  I also have an image of just  
the background.  Is there any way to use the image of the background  
to subtract the background from the original image using imagej?  I  
see a plugin that allows the program to figure out the background and  
subtract it from what it thinks the background is, but I can't find  
anything for if you know the background already.
Thank you,
Brittany Oliva

[ctrueden]

Hi Brittany,

If you load the background image into ImageJ along with your data, you can
subtract it by using the Image Calculator (in the Process menu).

-Curtis

On Mon, Aug 15, 2011 at 3:35 PM, Brittany Oliva <[hidden email]> wrote:

> Hello,
>    I have a question that I cannot find information for on the website.  I
> have an image which has a background that I want to get rid of, but it's an
> abnormal background.  I also have an image of just the background.  Is there
> any way to use the image of the background to subtract the background from
> the original image using imagej?  I see a plugin that allows the program to
> figure out the background and subtract it from what it thinks the background
> is, but I can't find anything for if you know the background already.
> Thank you,
> Brittany Oliva
>

[Gabriel Landini]

On Monday 15 Aug 2011, you wrote:
> Hello,
>      I have a question that I cannot find information for on the
> website.  I have an image which has a background that I want to get
> rid of, but it's an abnormal background.  I also have an image of just
> the background.  Is there any way to use the image of the background
> to subtract the background from the original image using imagej?  I
> see a plugin that allows the program to figure out the background and
> subtract it from what it thinks the background is, but I can't find
> anything for if you know the background already.

You do not specify what sort of imaging modality you are using, so  I am not
sure if this would help:

http://imagejdocu.tudor.lu/doku.php?id=howto:working:how_to_correct_background_illumination_in_brightfield_microscopy

If you search with google "background subtraction imagej" the first page
returns all useful links, including the above.

Regards
G