Brightness and contrast adjustment of single slice in stack

[Stan Bindon]

I have stacks with 16-bit images, all of which I would like to adjust
brightness and contrast while keeping them in the stack for convenience.
After searching the list, the solution posted in 2001 was to hold down alt
while turning on animation.

Is there an easier/more straightforward way to do this?

Thanks.

[Du, Guanghua]

Hi, Stan Bindon,
one way (maybe it's not a good way) to do this is first to save your
stacks as an image sequence, then adjust the contrast of each image,
after that, you import them into a stack.


Stan Bindon wrote:
> I have stacks with 16-bit images, all of which I would like to adjust
> brightness and contrast while keeping them in the stack for convenience.
> After searching the list, the solution posted in 2001 was to hold down alt
> while turning on animation.
>
> Is there an easier/more straightforward way to do this?
>
> Thanks.

--
best wishes!
**************************************
Guanghua Du,
********************************

[Wayne Rasband]

On Apr 1, 2009, at 6:55 AM, Stan Bindon wrote:

> I have stacks with 16-bit images, all of which I would like to adjust
> brightness and contrast while keeping them in the stack for  
> convenience.
> After searching the list, the solution posted in 2001 was to hold  
> down alt
> while turning on animation.
>
> Is there an easier/more straightforward way to do this?

Use the Image>Hyperstacks>Stack to Hyperstack command. In the dialog  
box, set "Channels:" to the number of images in the stack, "Slices:"  
to 1, "Frames:" to 1 and "Display Mode:" to "Grayscale".

-wayne