On Jul 6, 2009, at 5:16 AM, Graham Wright wrote:
> Hello,
>
> I have a problem with adjusting the brightness and contrast of a stack,
> unique to one computer. If you use Image>Adjust>Brightness/Contrast on
> a
> stack it will normally (on my other machines) adjust all the images in
> the
> stack the same way. However on this particular computer it is only
> adjusting
> the currently displayed slice of the stack, and not the other slices.
> The
> same data on a different computer with the same version of ImageJ
> works as i
> would expect it to.
>
> It's a Windows Vista Business SP1 machine, something tells me this
> could be
> the problem (!), running ImageJ v. 1.41o. I have downgraded and
> upgraded the
> software but the problem persists. The files in question are metamorph
> stk
> files, but the issue is present with some of the ImageJ sample files
> too.
>
> I'm guessing there's a box unchecked somewhere that i was unaware of,
> so any
> help will be much appreciated.
Is the stack opening as a "composite" image (e.g., the 5 channel
"Neuron" sample stack)? This is the only kind of stack that allows you
to separately adjust the brightness and contrast of each image. With
"composite" stacks, the v1.43c daily build adds a "Composite mode:
mode" line to the Image>Show Info output, where 'mode' is "composite",
"color" or "grayscale". Here is the output for the "Neuron" sample
stack:
Title: Rat_Hippocampal_Neuron.tif
Width: 81.92 µm (512)
Height: 81.92 µm (512)
Depth: 1.00 µm (1)
Resolution: 6.250 pixels per µm
ID: -5
Coordinate origin: 0,0,0
Bits per pixel: 16 (unsigned)
Display range: 472 - 2436
Image: 1/5
Channels: 5
Composite mode: "color"
No Threshold
Uncalibrated
Path: Rat_Hippocampal_Neuron.tif
No Selection
-wayne
Locked
