Calculate time decay constant

[Nicolás Pírez]

Hi all, I need to calculate the decay constant of a fluorescent response. I
was wondering if some could help me with that. Does ImageJ has a plugging
for analysis of temporal dynamics?

Thanks!

Nico.

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Nicolás Pírez, Ph.D.
[hidden email]
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[wirthvolker]

Hi,
I cannot offer practical experience, but maybe a starting point: You
could measure the intensity in a stack (of your time-lapse exposures)
and fit the values to an exponential decay
(http://rsbweb.nih.gov/ij/developer/macro/functions.html#Fit).
Here is illustrated help: http://www.macbiophotonics.ca/imagej/t.htm
Best regards,
Volker


2012/11/2 Nicolás Pírez <[hidden email]>:

> Hi all, I need to calculate the decay constant of a fluorescent response. I
> was wondering if some could help me with that. Does ImageJ has a plugging
> for analysis of temporal dynamics?
>
> Thanks!
>
> Nico.
>
> --
> Nicolás Pírez, Ph.D.
> [hidden email]
> [phone]
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Cammer, Michael]

A few years ago we used ImageJ for widefield time domain FLIM analysis as suggested below.  We simply measured the intensity through time and used the fit function for a single exponential.  The experimental changes were dramatic and fit close enough to a single exponential so we used this instead of multiple fits in Origin.

_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

________________________________________
From: ImageJ Interest Group [[hidden email]] on behalf of Volker Wirth [[hidden email]]
Sent: Friday, November 02, 2012 2:47 PM
To: [hidden email]
Subject: Re: Calculate time decay constant

Hi,
I cannot offer practical experience, but maybe a starting point: You
could measure the intensity in a stack (of your time-lapse exposures)
and fit the values to an exponential decay
(http://rsbweb.nih.gov/ij/developer/macro/functions.html#Fit).
Here is illustrated help: http://www.macbiophotonics.ca/imagej/t.htm
Best regards,
Volker


2012/11/2 Nicolás Pírez <[hidden email]>:

> Hi all, I need to calculate the decay constant of a fluorescent response. I
> was wondering if some could help me with that. Does ImageJ has a plugging
> for analysis of temporal dynamics?
>
> Thanks!
>
> Nico.
>
> --
> Nicolás Pírez, Ph.D.
> [hidden email]
> [phone]
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html