Put images from .JPEG and .lsm file together

[Fabiano Perina]

Hi everyone,
I've been tried put two images differents of the same sample, where one is from
a Nikon D7000 digital camera, take with a macro lens 60 mm and another image is a lsm file form a High speed confocal microscope and contains a z stack in two color (red and cyan, where I have 14 slices per channel).

The problem that I have to face is

1) First how the better way to do it? (I've tried use merge channels, make a 3d projection....)

2)The big problem is: even if I change the image from digital camera to the same
pattern  (width, hight, voxel depth,  canvas size, etc.) I still cannot put the images together because the useful size (from digital camera image) keeps small than the microscope confocal images (lsm files that contains 17 slices in each channels).

How can do it in the best way? Any ideas?

I would be very greatful if you could help me a little

with my best regards, Perina


--
                                           Fabiano J. Perina

                          PhD(c) - Federal University of Lavras - BRA
                                  University of Delaware, DE - USA

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Albert Cardona-2]

2012/11/2 Fabiano Perina <[hidden email]>

> Hi everyone,
> I've been tried put two images differents of the same sample, where one is
> from
> a Nikon D7000 digital camera, take with a macro lens 60 mm and another
> image is a lsm file form a High speed confocal microscope and contains a z
> stack in two color (red and cyan, where I have 14 slices per channel).
>
> The problem that I have to face is
>
> 1) First how the better way to do it? (I've tried use merge channels, make
> a 3d projection....)
>
> 2)The big problem is: even if I change the image from digital camera to
> the same
> pattern  (width, hight, voxel depth,  canvas size, etc.) I still cannot
> put the images together because the useful size (from digital camera image)
> keeps small than the microscope confocal images (lsm files that contains 17
> slices in each channels).
>
> How can do it in the best way? Any ideas?
>
> I would be very greatful if you could help me a little
>
> with my best regards, Perina
>

A quick comment: I would use "AND" in the "Paste control", so that when you
paste the confocal onto the regular image, the background parts of the
confocal are not inserted.
There are many ways to copy; explore the options in "Paste control".

Albert

--
http://albert.rierol.net
http://www.ini.uzh.ch/~acardona/

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Fabiano Perina]

Hi Albert
I'll try your advice, thank you so much for this tip!

Best wishes, Fabiano

>
>
>>
>> > Hi everyone,
>> > I've been tried put two images differents of the same sample, where one
>> is
>> > from
>> > a Nikon D7000 digital camera, take with a macro lens 60 mm and another
>> > image is a lsm file form a High speed confocal microscope and contains
>> a z
>> > stack in two color (red and cyan, where I have 14 slices per channel).
>> >
>> > The problem that I have to face is
>> >
>> > 1) First how the better way to do it? (I've tried use merge channels,
>> make
>> > a 3d projection....)
>> >
>> > 2)The big problem is: even if I change the image from digital camera to
>> > the same
>> > pattern  (width, hight, voxel depth,  canvas size, etc.) I still cannot
>> > put the images together because the useful size (from digital camera
>> image)
>> > keeps small than the microscope confocal images (lsm files that
>> contains 17
>> > slices in each channels).
>> >
>> > How can do it in the best way? Any ideas?
>> >
>> > I would be very greatful if you could help me a little
>> >
>> > with my best regards, Perina
>> >
>>
>>
>>
>> A quick comment: I would use "AND" in the "Paste control", so that when
>> you
>> paste the confocal onto the regular image, the background parts of the
>> confocal are not inserted.
>> There are many ways to copy; explore the options in "Paste control".
>>
>> Albert
>>
>> --
>> http://albert.rierol.net
>> http://www.ini.uzh.ch/~acardona/
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
>
>
> --
> *                                           **Fabiano J. Perina*
> *
> *
> *                          **PhD(c) - Federal University of Lavras - BRA**
>  *
> *                                  University of Delaware, DE - USA *
> *                           *
> *                        *
>
>
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html