Caluculate Ave and SD of cell membrane thickness

[M.M.M.]

Hello.

I have a image of single cell that is fluorescent labeled its cell membrane.
I want to know average and standard deviasion of cell membrane thickness from its fluorescence.
Please tell me how to.


Sorry for my poor English.
I beg your kindness.
thank you.

[reinhard rachel]

>>> 14.11.2012 um 01:45:
> I have a image of single cell that is fluorescent labeled its cell membrane.
> I want to know average and standard deviasion of cell membrane thickness
> from its fluorescence.
> Please tell me how to.

sorry, but from an image taken by fluorescence microscopy, you can only "see", i.e. detect the membrane, but you cannot determine the thickness of this membrane (if we are talking about a biological lipid bilayer membrane, with a thickness of about 6 to 8 nm). So far, this can only be determined = measured by high resolution techniques like electron microscopy or in special cases (isolated membranes) by AFM / scanning probe microscopy. The real resolution of the fluorescence signal is way too poor, still. (you may get the magnitude - it is in the range of 'little less than' 10 nm ...)
kind regards,
Reinhard

PS: dear "epigeneticboy" -- it is fair to also give your full name (and address), if you are addressing a question to the imageJ listserver --- my opinion.

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