Hi Giacomo,
if you can convert your image to a binary one showing the cell
walls, e.g. .by thresholding, you can use
Process>Binary>Distance Map ("EDM" for short) or
Process>Binary Ultimate Points. (see the documentation)
Especially, the value of the EDM at the pixels obtained by
Skeletonize might be interesting (skeletonize a duplicate of the
binary image, Create Selection, transfer it to the EDM by
Restore Selection. Them, the Mean obtained by the Measure
command should give you roughly half the thickness of the walls
in pixels.
Michael
________________________________________________________________
On 29 Nov 2007, at 09:47, Giacomo Goli wrote:
> Dear all,
> we are a laboratory of wood technology and we are quiet new to ImageJ.
> We arrived very easily to measure the vessels by the "analyse
> particles"
> function, and the cell wall thickness by the line tool and the
> measuring
> function.
> We are wondering if there is an automatic way to measure the cell wall
> thickness by drawing a line across one or more cell walls and obtain
> automatically the wall thickness or an average thickness of the
> crossed cell
> walls.
> Anybody can help with this??
> Thank you in advance.
> Giacomo Goli
>
>
>
> --
>
>
> __________________________________________________
> Giacomo Goli - Phd Dr.
> url:
http://www.giacomogoli.net> Dipartimento di Scienze e Tecnologie Ambientali Forestali
> Università di Firenze
>
http://www.laurealegno.unifi.it> Via S. Bonaventura 13 - 50145 Firenze ITALIA
> tel: +390553288675
> cell: +393290656674
> fax: +39055319179
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