Change ROI size

[Benito Alarcon]

Hello,
I want to measure the distribution of  a
protein-RFP in the neighbourhood of the nuclear
menbrane. I can make a ROI of the nucleous
(thanks to a nuclear mark) by the polygon tool
(the nucleous is irregular somehow). In order to
measure if the protein-RFP change its
distribution around the nuclear membrane, I think
that a way could be via ROIs with certain amount
bigger or smaller (a percentage or númber of
pixels) than the ROI of the nucleous. Is this possible by ImageJ?
thanks a lot
Benito Alarcon
Instituto de Biomedicina de Valencia
Valencia.Spain

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[Michael Schmid]

Hi Benito,

seems you are looking for Edit>Selection>Enlarge
  http://rsb.info.nih.gov/ij/docs/guide/146-27.html#toc-Subsubsection-27.12.14

Michael
________________________________________________________________
On Oct 1, 2014, at 10:00, Benito Alarcon wrote:

> Hello,
> I want to measure the distribution of  a protein-RFP in the neighbourhood of the nuclear menbrane. I can make a ROI of the nucleous (thanks to a nuclear mark) by the polygon tool (the nucleous is irregular somehow). In order to measure if the protein-RFP change its distribution around the nuclear membrane, I think that a way could be via ROIs with certain amount bigger or smaller (a percentage or númber of pixels) than the ROI of the nucleous. Is this possible by ImageJ?
> thanks a lot
> Benito Alarcon
> Instituto de Biomedicina de Valencia
> Valencia.Spain

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