Change pixel resolution

[Weller Andrew Francis]

Dear all,

When I run this command it includes particles that are too small (I
don't need):

run("Analyze Particles...", "size=0.5-Infinity circularity=0.00-1.00
show=Masks clear include");

So I change the size to something silly like "size=500", but it appears
that this is too big - it shouldn't be!

When I check my images, the resolution appears to say "3.84x2.88
(768x576)"- strange?! My images are 'really' 768x576 not 3.84x2.88 and I
feel that run("Analyze Particles...") is working on the wrong
resolution.

How can I implement a pixel resolution conversion within my macro so
that I open an image and the 1st thing I do is tell it to use the
768x576 resolution, not the 3.84x2.88 resolution? For example:

open(image);
CHANGE RESOLUTION TO 768x576
do imaging stuff...

Many thanks, Andy

[Vincenzo Della Mea]

You perhaps have set the scale (with Analyze:Set Scale) some time  
ago, choosing "Global".  Change it.
Vincenzo

> When I check my images, the resolution appears to say "3.84x2.88
> (768x576)"- strange?! My images are 'really' 768x576 not 3.84x2.88  
> and I
> feel that run("Analyze Particles...") is working on the wrong
> resolution.

[Wayne Rasband]

To change the scale to pixels enter zero as the "Distance in Pixels"  
in the Analyze>Set Scale dialog. In a macro, use

      run("Set Scale...", "distance=0");

Check "Global" in the dialog to use a pixel scale for all images  
opened during the current session, or use

     run("Set Scale...", "distance=0 global");

in a macro

-wayne

On Jul 4, 2006, at 5:11 AM, Andy Weller wrote:

> Dear all,
>
> When I run this command it includes particles that are too
> small (I don't need):
>
> run("Analyze Particles...", "size=0.5-Infinity circularity=0.00-1.00
> show=Masks clear include");
>
> So I change the size to something silly like "size=500",
> but it appears that this is too big - it shouldn't be!
>
> When I check my images, the resolution appears to say "3.84x2.88
> (768x576)"- strange?! My images are 'really' 768x576 not 3.84x2.88
> and I feel that run("Analyze Particles...") is working on the wrong
> resolution.
>
> How can I implement a pixel resolution conversion within my
> macro so that I open an image and the 1st thing I do is tell it
> to use the 768x576 resolution, not the 3.84x2.88 resolution? For  
> example:
>
> open(image);
> CHANGE RESOLUTION TO 768x576
> do imaging stuff...
>
> Many thanks, Andy