Changing the hue of an image

[Linda Barthel-2]

I have an 8 bit grayscale image, .tif.  Want to change it to a color image,
with a "grayish" blue hue.  Very easy to convert to straight blue-but that
isn't the best color.
Thanks,
L

--
*Linda Barthel M.Sc.  *
Lead Product Scientist-Cytogenomics - myTags
Office: 1 [phone] <1-734-388-8695>
Cell: 1 [phone] <1-734-355-4771>

5840 Interface Drive, Suite 101 | Ann Arbor, MI, 48103, USA
Web www.arborbiosci.com | Twitter @ArborBio <http://twitter.com/ArborBio>

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[Jeremy Adler]

Hej Linda,

This should be quite simple

Start with an image
Then
Image/color/edit LUT
A window appears that allows you to edit the  a LUT
With the mouse select the top left square and end with the bottom right square

The software asks you for the RGB combination for the top left square (lowest value)
Maybe 0,0,0               which be  black
Then the combination for the last (most intense) square
Maybe 128,128,255
Blue set to 255 and R & G to 128, a blue plus grey -
All the intermediate values are interpolated.

Save the new LUT in Imagej's   LUTS folder and restart.
The LUT will now be available.


Jeremy Adler
===============================================
                    B i o V i s   P l a t f o r m of  Uppsala University
                   Light & EM microscopy / FlowCytometry & Cell Sorting / Image Analysis
===============================================
Jeremy Adler   PhD - Senior research engineer
Light, Confocal Microscopy, Image Analysis
E-mail: [hidden email]
070-1679349

Dag Hammarskjölds v 20
751 85 UPPSALA, SWEDEN
http://biovis.uu.se/
===============================================








-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Linda Barthel
Sent: Friday, February 14, 2020 10:24 PM
To: [hidden email]
Subject: Changing the hue of an image

I have an 8 bit grayscale image, .tif.  Want to change it to a color image, with a "grayish" blue hue.  Very easy to convert to straight blue-but that isn't the best color.
Thanks,
L

--
*Linda Barthel M.Sc.  *
Lead Product Scientist-Cytogenomics - myTags
Office: 1 [phone] <1-734-388-8695>
Cell: 1 [phone] <1-734-355-4771>

5840 Interface Drive, Suite 101 | Ann Arbor, MI, 48103, USA Web www.arborbiosci.com | Twitter @ArborBio <http://twitter.com/ArborBio>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html








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[Krs5]

Dear Linda

Maybe the Cyan LUT is working for you (Image > Lookup Tables> Cyan). Or you can take any LUT, select Image > Color >Edit LUT... and select all the fields in the Editor. It will then ask you the start values for Red, Green and Blue and after 'OK' for the end Values for Green, Red and Blue and then fill in the gradient for the LUT automatically, so you can create any LUT to your own taste.

Best wishes

Kees


Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
www.le.ac.uk/advanced-imaging-facility


-----Original Message-----
From: ImageJ Interest Group <[hidden email]> On Behalf Of Linda Barthel
Sent: 14 February 2020 21:24
To: [hidden email]
Subject: Changing the hue of an image

I have an 8 bit grayscale image, .tif.  Want to change it to a color image, with a "grayish" blue hue.  Very easy to convert to straight blue-but that isn't the best color.
Thanks,
L

--
*Linda Barthel M.Sc.  *
Lead Product Scientist-Cytogenomics - myTags
Office: 1 [phone] <1-734-388-8695>
Cell: 1 [phone] <1-734-355-4771>

5840 Interface Drive, Suite 101 | Ann Arbor, MI, 48103, USA Web https://eur03.safelinks.protection.outlook.com/?url=www.arborbiosci.com&amp;data=02%7C01%7Ckrs5%40leicester.ac.uk%7C183f9dde89754c3121fb08d7b4290429%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C1%7C637175960586285626&amp;sdata=y%2FnfhPI2xCXrZcgTeir%2BgRNls8JXRmfVZQhlmWBAVQc%3D&amp;reserved=0 | Twitter @ArborBio <https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Ftwitter.com%2FArborBio&amp;data=02%7C01%7Ckrs5%40leicester.ac.uk%7C183f9dde89754c3121fb08d7b4290429%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C1%7C637175960586285626&amp;sdata=g0BXZLJGH4sOd6ucz3IW7vF4m4YprTqh%2F1MrlafdnKI%3D&amp;reserved=0>

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[Linda Barthel-2]

Thanks Jeremy-worked perfectly.  I used 115-red, 175-green, 255-blue to get
the desired hue.
Regards,
Linda

On Tue, Feb 18, 2020 at 3:40 AM Jeremy Adler <[hidden email]> wrote:

> Hej Linda,
>
> This should be quite simple
>
> Start with an image
> Then
> Image/color/edit LUT
> A window appears that allows you to edit the  a LUT
> With the mouse select the top left square and end with the bottom right
> square
>
> The software asks you for the RGB combination for the top left square
> (lowest value)
> Maybe 0,0,0               which be  black
> Then the combination for the last (most intense) square
> Maybe 128,128,255
> Blue set to 255 and R & G to 128, a blue plus grey -
> All the intermediate values are interpolated.
>
> Save the new LUT in Imagej's   LUTS folder and restart.
> The LUT will now be available.
>
>
> Jeremy Adler
> ===============================================
>                     B i o V i s   P l a t f o r m of  Uppsala University
>                    Light & EM microscopy / FlowCytometry & Cell Sorting /
> Image Analysis
> ===============================================
> Jeremy Adler   PhD - Senior research engineer
> Light, Confocal Microscopy, Image Analysis
> E-mail: [hidden email]
> 070-1679349
>
> Dag Hammarskjölds v 20
> 751 85 UPPSALA, SWEDEN
> http://biovis.uu.se/
> ===============================================
>
>
>
>
>
>
>
>
> -----Original Message-----
> From: ImageJ Interest Group <[hidden email]> On Behalf Of Linda
> Barthel
> Sent: Friday, February 14, 2020 10:24 PM
> To: [hidden email]
> Subject: Changing the hue of an image
>
> I have an 8 bit grayscale image, .tif.  Want to change it to a color
> image, with a "grayish" blue hue.  Very easy to convert to straight
> blue-but that isn't the best color.
> Thanks,
> L
>
> --
> *Linda Barthel M.Sc.  *
> Lead Product Scientist-Cytogenomics - myTags
> Office: 1 [phone] <1-734-388-8695>
> Cell: 1 [phone] <1-734-355-4771>
>
> 5840 Interface Drive, Suite 101 | Ann Arbor, MI, 48103, USA Web
> www.arborbiosci.com | Twitter @ArborBio <http://twitter.com/ArborBio>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
>
>
>
>
>
>
>
> När du har kontakt med oss på Uppsala universitet med e-post så innebär
> det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör
> det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/
>
> E-mailing Uppsala University means that we will process your personal
> data. For more information on how this is performed, please read here:
> http://www.uu.se/en/about-uu/data-protection-policy
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
*Linda Barthel M.Sc.  *
Lead Product Scientist-Cytogenomics - myTags
Office: 1 [phone] <1-734-388-8695>
Cell: 1 [phone] <1-734-355-4771>

5840 Interface Drive, Suite 101 | Ann Arbor, MI, 48103, USA
Web www.arborbiosci.com | Twitter @ArborBio <http://twitter.com/ArborBio>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html