Channel removal question

[Guglielmo Zanghi]

Hello,

I need to merge the blue channel with the green channel (with two epifluorescence microscope images), but rather than adding what is seen on both image, to remove what is seen on the green channel in the blue channel, to confirm the presence of cells.

How can I do that?

Thank you very much
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[GuyM]

Hi
If I understand correctly, this is a simple subtraction operation, then why not use the image calculator (In the main menu: Process, then choose Image calculator)? You can choose the channel you want to subtract from the other.
Good luck
Guy

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[Guglielmo Zanghi]

Hello,

I have just tried your suggestion, it seems to be what I required, thank you very much for your help!

Guglielmo

> On 11 Aug 2015, at 13:30, Guy <[hidden email]> wrote:
>
> Hi
> If I understand correctly, this is a simple subtraction operation, then why not use the image calculator (In the main menu: Process, then choose Image calculator)? You can choose the channel you want to subtract from the other.
> Good luck
> Guy
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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