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Hi all,
I have two images that I have to logarithmically transform and invert each, and then I have to subtract one image from the other.
To transform I have used process>math>log and to invert I used edit>invert.
The images do not look inverted after I do this, and the mean pixel value on my ROI is exactly the same after each process as it was on the original images, is that correct?
I know that invert LUT inverts the colour but I need the pixel values inverted.
I ask because when I subtract the images I get a completely black image and I'm trying to work out why.
Very confused imageJ novice.
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